Rat Nephritogenic Monoclonal Antibodies to Glomerular Basement Membrane
ثبت نشده
چکیده
A novel rat model of nephritis induced by monoclonal antibodies to the non-collagenous domain of type IV collagen was developed by Sado’s group [1-2]. Chondrex, Inc. provides three rat monoclonal antibodies to non-collagenous domain 1 (NC1) of α4 chain of type IV collagen, α4(IV), of rat glomerular basement membrane (GBM), which are used for inducing nephritis in Wistar-Kyoto rats by a single intraperitoneal (IP) or intravenous (IV) injection.
منابع مشابه
Isolation and characterization of the nephritogenic antigen producing anti-tubular basement membrane disease
Using monoclonal antibody affinity chromatography, we isolated a 48,000 mol wt, glucose-rich glycoprotein (3M-1) from collagenase-solubilized rabbit renal tubular basement membrane (SRTA). The purified 3M-1 protein is noncollagenous, and is capable of inducing anti-TBM (tubular basement membrane) antibodies and interstitial nephritis in susceptible hosts. Further, when SRTA, at a normally nephr...
متن کاملProduction and immunohistochemical characterization of monoclonal antibodies directed against renal basement membranes of rats.
Basement membranes were separated from rat glomeruli and purified by mild procedures, which led to a highly enriched basement membrane fraction. Here, the production and characterization of five monoclonal antibodies against tubular and glomerular basement membranes are described. These antibodies were analyzed immunohistochemically on frozen sections of rat, bovine, and human kidneys as well a...
متن کاملAntigen specificity of anti-nuclear antibodies complexed to nucleosomes determines glomerular basement membrane binding in vivo.
Monoclonal anti-nuclear antibodies which are complexed to nucleosomes are able to bind to the glomerular basement membrane (GBM) in vivo, whereas purified antibodies do not bind. The positively charged histone moieties in the nucleosome are-responsible for the binding to anionic determinants in the GBM. We tested the hypothesis that the specificity of the autoantibodies complexed to the nucleos...
متن کاملAnti-GBM glomerulonephritis: a T cell-mediated autoimmune disease?
Anti-glomerular basement membrane (GBM) glomerulonephritis, which was among the earliest recognized human autoimmune diseases, is characterized by the presence of anti-GBM antibody. It has been a prototypical example of autoantibody-mediated autoimmune disease. However, decades of research on this disease, based either on clinical observations or experimental models, have revealed that T cell-m...
متن کاملSusceptibility to anti-glomerular basement membrane disease and Goodpasture syndrome is linked to MHC class II genes and the emergence of T cell-mediated immunity in mice.
We developed a new mouse model of human anti-glomerular basement membrane (GBM) disease to better characterize the genetic determinants of cell-mediated injury. While all major histocompatibility complex (MHC) haplotypes (H-2a, k, s, b, and d) immunized with alpha3 NC1 domains of type IV collagen produce anti-alpha3(IV) NC1 antibodies that cross-react with human Goodpasture [anti-GBM/anti-alpha...
متن کامل